 February 2016 |
The expression data set has been updated. A new embryonic stage "4-cell" was added from 250 handpicked 4-cell stage embryos. An aged adult sample ("sterile adult") was added from synchronized spe-9(hc88) L1 worms cultured at 23°C for 8 days after L4 molt. The spe-9(hc88) animals have defective sperm and fail to fertilize oocytes. An "adult gonad" sample was added from disected gonads. We made minor adjustments to our algorithm that combines the different embryonic times series to estimate expression at 17 different stages. These algorithmic changes have resulted in minor differences in expression values from those in the previous version. In addition we anchored the imputed times to the 0223 series, which was judged to be the most complete and consistent. The resultant time points are in reasonable agreement with other data sets, based on overall gene expression patterns. The 17 old embryonic time points map to the new ones as follows: 38 min -> 44 min, 85 -> 83, 131 ->122, 178 -> 161, 224 -> 199, 271 -> 238, 318 -> 277, 364 -> 316, 411 -> 355, 457 -> 393, 504 -> 432, 551 -> 471, 597 -> 510, 644 -> 548, 690 -> 587, 737 -> 626, 784 -> 665 |